Cellular and Molecular Biomechanics Laboratory

The laboratory of Dr. Cheng Zhu at Georgia Tech

Please look at available positions.

Recent Publications

Type Date Title Link
9/27/2017 Receptor-mediated cell mechanosensing. Full
9/11/2017 Neutrophil FcγRIIA promotes IgG-mediated glomerular neutrophil capture via Abl/Src kinases. Full
5/12/2017 L-selectin mechanochemistry restricts neutrophil priming in vivo. Full
3/6/2017 In situ and in silico kinetic analyses of programmed cell death-1 (PD-1) receptor, programmed cell death ligands, and B7-1 protein interaction network. Full
3/3/2017 Two-Dimensional Analysis of Cross-Junctional Molecular Interaction by Force Probes. Full

Positions

Graduate Research Assistant (GRA) and Postdoctoral Scholar positions are immediately available for qualified and highly motivated individuals to work on the following projects funded by the NIH or NSF (click at the hyperlinks to view the abstracts of the grants):


Please email Dr. Cheng Zhu for more information.

People

Principal Investigator

Dr. Cheng Zhu

cheng.zhu@bme.gatech.edu

Post-doctorates

Dr. Paul Cardenas-Lizana

pacl3@gatech.edu

Dr. Hyun-Jung Lee

hjlee@gatech.edu

Dr. Kaitao Li

KaitaoLi@gatech.edu

Graduate Students

Fletcher Griffitts

griffitts@gatech.edu

Jiexi Liao

jliao8@gatech.edu

Steve Park

Aaron Rosado

arosado@gatech.edu

Muaz Rushdi

nmnr3@gatech.edu

Fangyuan Zhou

fzhou22@gatech.edu

Visiting Scholars

Laboratory Alumni

Laboratory Staff

Research

Welcome!

Welcome to the Zhu lab. We study single molecular adhesion with force spectroscopy for various cells and disease models. We pioneered in the discovery of force-prolonged bond lifetime, or the "catch bond," using the biomembrane force probe paired with mutation studies and molecular dynamics simulations.

T Cell Development, Antigen Recognition, and Activation

T cells are an important subset of the immune system that plays a pivotal role in regulating various diseases. T cells functions are influenced both by biochemical and biophysical properties at local microenvironment that influences receptor-ligand interactions. Our goal is to understand how these interactions mediated by mechanical properties translate into intracellular signaling, thereby affecting cellular function and fate.

Platelet Mechanobiology

Platelets are key players in both hemostasis and thrombosis. Mechanical stimuli are important in the circulation system. We strive to understand platelet activation, mechanotransduction, and hyperreactivity by characterizing surface receptor-ligand binding kinetics and intracellular signaling using tools such as the biomembrane force probe, biosensors, and mutation studies.

Instruments

Biomembrane Force Probe

BFP

Measures single ligand-receptor interactions under force

Micropipette adhesion frequency assay

Measures adhesion frequency of single receptor-ligand interactions

Atomic force microscope

AFM

Home-made AFM that measures protein-protein binding kinetics

Horizontal atomic force microscope

HAFM

AFM on which the cantilever works horizontally (parallel to the earth); can measure receptor-ligand binding kinetics under force on live cells

Micropipette forge

Home-made micropipette factory: makes pipettes of various sizes

Publications

Title First Author Journal Date PMID DOI More Information
Receptor-mediated cell mechanosensing. Yunfeng Chen Molecular biology of the cell 9/27/2017 28954860 10.1091/mbc.E17-04-0228 More Info
Neutrophil FcγRIIA promotes IgG-mediated glomerular neutrophil capture via Abl/Src kinases. Hiroshi Nishi The Journal of clinical investigation 9/11/2017 28891817 10.1172/JCI94039 More Info
L-selectin mechanochemistry restricts neutrophil priming in vivo. Zhenghui Liu Nature communications 5/12/2017 28497779 10.1038/ncomms15196 More Info
In situ and in silico kinetic analyses of programmed cell death-1 (PD-1) receptor, programmed cell death ligands, and B7-1 protein interaction network. Kaitao Li The Journal of biological chemistry 3/6/2017 28270509 10.1074/jbc.M116.763888 More Info
Two-Dimensional Analysis of Cross-Junctional Molecular Interaction by Force Probes. Lining Ju Methods in molecular biology (Clifton, N.J.) 3/3/2017 28255706 10.1007/978-1-4939-6881-7_15 More Info
Notch-Jagged complex structure implicates a catch bond in tuning ligand sensitivity. Vincent C Luca Science (New York, N.Y.) 3/2/2017 28254785 10.1126/science.aaf9739 More Info
The integrin PSI domain has an endogenous thiol isomerase function and is a novel target for antiplatelet therapy. Guangheng Zhu Blood 1/25/2017 28122739 10.1182/blood-2016-07-729400 More Info
Glycan Bound to the Selectin Low Affinity State Engages Glu-88 to Stabilize the High Affinity State under Force. Padmaja Mehta-D'souza The Journal of biological chemistry 12/23/2016 28011641 10.1074/jbc.M116.767186 More Info
Cooperative unfolding of distinctive mechanoreceptor domains transduces force into signals. Lining Ju eLife 7/19/2016 27434669 10.7554/eLife.15447 More Info
Force regulated conformational change of integrin αVβ3. Yunfeng Chen Matrix biology : journal of the International Society for Matrix Biology 7/14/2016 27423389 10.1016/j.matbio.2016.07.002 More Info

Contact Zhu Lab

If you would like to contact the laboratory of Dr. Cheng Zhu, please get in touch with Larissa (E-mail)

For information about web design or web application design used in this website please contact Aaron (E-mail)

The website is administrated by Jiexi Liao (E-mail)