Cellular and Molecular Biomechanics Laboratory

The laboratory of Dr. Cheng Zhu at Georgia Tech

Recent Publications

Type Date Title Link
9/2/2015 Force-Regulated In Situ TCR-Peptide-Bound MHC Class II Kinetics Determine Functions of CD4+ T Cells. Full
8/4/2015 Fluorescence Biomembrane Force Probe: Concurrent Quantitation of Receptor-ligand Kinetics and Binding-induced Intracellular Signaling on a Single Cell Full
6/20/2015 Von Willebrand factor-A1 domain binds platelet glycoprotein Ibα in multiple states with distinctive force-dependent dissociation kinetics. Full
6/8/2015 Pre-TCR ligand binding impacts thymocyte development before αβTCR expression. Full
5/22/2015 The cellular environment regulates in situ kinetics of T-cell receptor interaction with peptide major histocompatibility complex. Full

People

Principal Investigator

Dr. Cheng Zhu

cheng.zhu@bme.gatech.edu

Post-doctorates

Dr. Hyun-Jung Lee

hjlee@gatech.edu

Graduate Students

Visiting Scholars

Laboratory Alumni

Dr. Jingsung Hong

hjs0811@gmail.com

Laboratory Staff

Research

Welcome!

Welcome to the Zhu lab. We study single molecular adhesion with force spectroscopy for various cells and disease models. We pioneered in the discovery of force-prolonged bond lifetime, or the "catch bond," using the biomembrane force probe paired with mutation studies and molecular dynamics simulations.

T Cell Development, Antigen Recognition, and Activation

T cells are an important subset of the immune system that plays a pivotal role in regulating various diseases. T cells functions are influenced both by biochemical and biophysical properties at local microenvironment that influences receptor-ligand interactions. Our goal is to understand how these interactions mediated by mechanical properties translate into intracellular signaling, thereby affecting cellular function and fate.

Platelet Mechanobiology

Platelets are key players in both hemostasis and thrombosis. Mechanical stimuli are important in the circulation system. We strive to understand platelet activation, mechanotransduction, and hyperreactivity by characterizing surface receptor-ligand binding kinetics and intracellular signaling using tools such as the biomembrane force probe, biosensors, and mutation studies.

Instruments

Biomembrane Force Probe

BFP

Measures single ligand-receptor interactions under force

Micropipette adhesion frequency assay

Measures adhesion frequency of single receptor-ligand interactions

Atomic force microscope

AFM

Home-made AFM that measures protein-protein binding kinetics

Horizontal atomic force microscope

HAFM

AFM on which the cantilever works horizontally (parallel to the earth); can measure receptor-ligand binding kinetics under force on live cells

Micropipette forge

Home-made micropipette factory: makes pipettes of various sizes

Publications

Title First Author Journal Date PMID DOI More Information
Force-Regulated In Situ TCR-Peptide-Bound MHC Class II Kinetics Determine Functions of CD4+ T Cells. Jinsung Hong Journal of immunology (Baltimore, Md. : 1950) 9/2/2015 26336148 10.4049/jimmunol.1501407 More Info
Fluorescence Biomembrane Force Probe: Concurrent Quantitation of Receptor-ligand Kinetics and Binding-induced Intracellular Signaling on a Single Cell Yunfeng Chen J Vis Exp. 8/4/2015 26274371 10.3791/52975 More Info
Von Willebrand factor-A1 domain binds platelet glycoprotein Ibα in multiple states with distinctive force-dependent dissociation kinetics. Lining Ju Thrombosis research 6/20/2015 26213126 10.1016/j.thromres.2015.06.019 More Info
Pre-TCR ligand binding impacts thymocyte development before αβTCR expression. Robert J Mallis Proceedings of the National Academy of Sciences of the United States of America 6/8/2015 26056289 10.1073/pnas.1504971112 More Info
The cellular environment regulates in situ kinetics of T-cell receptor interaction with peptide major histocompatibility complex. Baoyu Liu European journal of immunology 5/22/2015 25944482 10.1002/eji.201445358 More Info
Transport regulation of two-dimensional receptor-ligand association. Lining Ju Biophysical journal 4/12/2015 25863068 10.1016/j.bpj.2015.02.023 More Info
A Lupus-Associated Mac-1 Variant Has Defects in Integrin Allostery and Interaction with Ligands under Force. Florencia Rosetti Cell reports 3/11/2015 25772353 10.1016/j.celrep.2015.02.037 More Info
Molecular force spectroscopy on cells. Baoyu Liu Annual review of physical chemistry 1/12/2015 25580628 10.1146/annurev-physchem-040214-121742 More Info
Ligand-engaged TCR is triggered by Lck not associated with CD8 coreceptor. Javier Casas Nature communications 11/27/2014 25427562 10.1038/ncomms6624 More Info
DNA-based digital tension probes reveal integrin forces during early cell adhesion. Yun Zhang Nature communications 10/24/2014 25342432 10.1038/ncomms6167 More Info

Contact Zhu Lab

If you would like to contact the laboratory of Dr. Cheng Zhu, please get in touch with Larissa (E-mail)

For information about web design or web application design used in this website please contact Aaron (E-mail)

The website is administrated by Jiexi Liao (E-mail)