Welcome to the Zhu lab. We study single molecular adhesion with force spectroscopy for various cells and disease models. We pioneered in the discovery of force-prolonged bond lifetime, or the "catch bond," using the biomembrane force probe paired with mutation studies and molecular dynamics simulations.
T cells are an important subset of the immune system that plays a pivotal role in regulating various diseases. T cells functions are influenced both by biochemical and biophysical properties at local microenvironment that influences receptor-ligand interactions. Our goal is to understand how these interactions mediated by mechanical properties translate into intracellular signaling, thereby affecting cellular function and fate.
Platelets are key players in both hemostasis and thrombosis. Mechanical stimuli are important in the circulation system. We strive to understand platelet activation, mechanotransduction, and hyperreactivity by characterizing surface receptor-ligand binding kinetics and intracellular signaling using tools such as the biomembrane force probe, biosensors, and mutation studies.
|Title||First Author||Journal||Date||PMID||DOI||More Information|
|Receptor-mediated cell mechanosensing.||Yunfeng Chen||Molecular biology of the cell||9/27/2017||28954860||10.1091/mbc.E17-04-0228||More Info|
|Neutrophil FcγRIIA promotes IgG-mediated glomerular neutrophil capture via Abl/Src kinases.||Hiroshi Nishi||The Journal of clinical investigation||9/11/2017||28891817||10.1172/JCI94039||More Info|
|L-selectin mechanochemistry restricts neutrophil priming in vivo.||Zhenghui Liu||Nature communications||5/12/2017||28497779||10.1038/ncomms15196||More Info|
|In situ and in silico kinetic analyses of programmed cell death-1 (PD-1) receptor, programmed cell death ligands, and B7-1 protein interaction network.||Kaitao Li||The Journal of biological chemistry||3/6/2017||28270509||10.1074/jbc.M116.763888||More Info|
|Two-Dimensional Analysis of Cross-Junctional Molecular Interaction by Force Probes.||Lining Ju||Methods in molecular biology (Clifton, N.J.)||3/3/2017||28255706||10.1007/978-1-4939-6881-7_15||More Info|
|Notch-Jagged complex structure implicates a catch bond in tuning ligand sensitivity.||Vincent C Luca||Science (New York, N.Y.)||3/2/2017||28254785||10.1126/science.aaf9739||More Info|
|The integrin PSI domain has an endogenous thiol isomerase function and is a novel target for antiplatelet therapy.||Guangheng Zhu||Blood||1/25/2017||28122739||10.1182/blood-2016-07-729400||More Info|
|Glycan Bound to the Selectin Low Affinity State Engages Glu-88 to Stabilize the High Affinity State under Force.||Padmaja Mehta-D'souza||The Journal of biological chemistry||12/23/2016||28011641||10.1074/jbc.M116.767186||More Info|
|Cooperative unfolding of distinctive mechanoreceptor domains transduces force into signals.||Lining Ju||eLife||7/19/2016||27434669||10.7554/eLife.15447||More Info|
|Force regulated conformational change of integrin αVβ3.||Yunfeng Chen||Matrix biology : journal of the International Society for Matrix Biology||7/14/2016||27423389||10.1016/j.matbio.2016.07.002||More Info|