Platelet is a key player in both hemostasis and thrombosis. Mechanical stimuli are being identified as important as biochemical cues in the hemodynamic environment of the circulation system. We are solving the puzzles of platelet activation and hyperreactivity by characterizing platelet receptor binding kinetics and signal transduction under forces.
T lymphocytes display a remarkable ability to evaluate differences in MHC-peptide complexes. Alteration of a single amino acid in an antigenic peptide has significant and distinct biological effects on the T cell recognition. Current research proposes all these events are governed by the TCR-MHC interactions. Using well developed micropipette and confocal microscope techniques, as well as relative cellular and mouse model, specific experiments are being conducted to find the common mechanism of the TCR-MHC interaction by quantitatively measuring the binding kinetics and comparing with corresponding biological effects, thereby providing a basis for rational design of immunotherapy.
AFM on which the cantilever works horizontally (parallel to the earth); can measure receptor-ligand binding kinetics under force on live cells
|Title||First Author||Journal||Date||PMID||DOI||More Information|
|Fluorescence Biomembrane Force Probe: Concurrent Quantitation of Receptor-ligand Kinetics and Binding-induced Intracellular Signaling on a Single Cell||Yunfeng Chen||J Vis Exp.||8/4/2015||26274371||10.3791/52975||More Info|
|Von Willebrand factor-A1 domain binds platelet glycoprotein Ibα in multiple states with distinctive force-dependent dissociation kinetics.||Lining Ju||Thrombosis research||6/20/2015||26213126||10.1016/j.thromres.2015.06.019||More Info|
|Pre-TCR ligand binding impacts thymocyte development before αβTCR expression.||Robert J Mallis||Proceedings of the National Academy of Sciences of the United States of America||6/8/2015||26056289||10.1073/pnas.1504971112||More Info|
|The cellular environment regulates in situ kinetics of T-cell receptor interaction with peptide major histocompatibility complex.||Baoyu Liu||European journal of immunology||5/22/2015||25944482||10.1002/eji.201445358||More Info|
|Transport regulation of two-dimensional receptor-ligand association.||Lining Ju||Biophysical journal||4/12/2015||25863068||10.1016/j.bpj.2015.02.023||More Info|
|A Lupus-Associated Mac-1 Variant Has Defects in Integrin Allostery and Interaction with Ligands under Force.||Florencia Rosetti||Cell reports||3/11/2015||25772353||10.1016/j.celrep.2015.02.037||More Info|
|Molecular force spectroscopy on cells.||Baoyu Liu||Annual review of physical chemistry||1/12/2015||25580628||10.1146/annurev-physchem-040214-121742||More Info|
|Ligand-engaged TCR is triggered by Lck not associated with CD8 coreceptor.||Javier Casas||Nature communications||11/27/2014||25427562||10.1038/ncomms6624||More Info|
|DNA-based digital tension probes reveal integrin forces during early cell adhesion.||Yun Zhang||Nature communications||10/24/2014||25342432||10.1038/ncomms6167||More Info|
|A generalizable, tunable microfluidic platform for delivering fast temporally varying chemical signals to probe single-cell response dynamics.||Loice Chingozha||Analytical chemistry||10/10/2014||25254360||10.1021/ac5019843||More Info|