Welcome to the Zhu lab. We study single molecular adhesion with force spectroscopy for various cells and disease models. We pioneered in the discovery of force-prolonged bond lifetime, or the "catch bond," using the biomembrane force probe paired with mutation studies and molecular dynamics simulations.
T cells are an important subset of the immune system that plays a pivotal role in regulating various diseases. T cells functions are influenced both by biochemical and biophysical properties at local microenvironment that influences receptor-ligand interactions. Our goal is to understand how these interactions mediated by mechanical properties translate into intracellular signaling, thereby affecting cellular function and fate.
Platelets are key players in both hemostasis and thrombosis. Mechanical stimuli are important in the circulation system. We strive to understand platelet activation, mechanotransduction, and hyperreactivity by characterizing surface receptor-ligand binding kinetics and intracellular signaling using tools such as the biomembrane force probe, biosensors, and mutation studies.
|Title||First Author||Journal||Date||PMID||DOI||More Information|
|Mechanical regulation of a molecular clutch defines force transmission and transduction in response to matrix rigidity.||Alberto Elosegui-Artola||Nature cell biology||4/11/2016||27065098||10.1038/ncb3336||More Info|
|Force-Induced Unfolding of Leucine-Rich Repeats of Glycoprotein Ibα Strengthens Ligand Interaction.||Lining Ju||Biophysical journal||11/5/2015||26536255||10.1016/j.bpj.2015.08.050||More Info|
|Regulatory and T Effector Cells Have Overlapping Low to High Ranges in TCR Affinities for Self during Demyelinating Disease.||Jennifer D Hood||Journal of immunology (Baltimore, Md. : 1950)||9/18/2015||26385521||10.4049/jimmunol.1501464||More Info|
|Force-Regulated In Situ TCR-Peptide-Bound MHC Class II Kinetics Determine Functions of CD4+ T Cells.||Jinsung Hong||Journal of immunology (Baltimore, Md. : 1950)||9/2/2015||26336148||10.4049/jimmunol.1501407||More Info|
|Fluorescence Biomembrane Force Probe: Concurrent Quantitation of Receptor-ligand Kinetics and Binding-induced Intracellular Signaling on a Single Cell||Yunfeng Chen||J Vis Exp.||8/4/2015||26274371||10.3791/52975||More Info|
|Von Willebrand factor-A1 domain binds platelet glycoprotein Ibα in multiple states with distinctive force-dependent dissociation kinetics.||Lining Ju||Thrombosis research||6/20/2015||26213126||10.1016/j.thromres.2015.06.019||More Info|
|Pre-TCR ligand binding impacts thymocyte development before αβTCR expression.||Robert J Mallis||Proceedings of the National Academy of Sciences of the United States of America||6/8/2015||26056289||10.1073/pnas.1504971112||More Info|
|The cellular environment regulates in situ kinetics of T-cell receptor interaction with peptide major histocompatibility complex.||Baoyu Liu||European journal of immunology||5/22/2015||25944482||10.1002/eji.201445358||More Info|
|Transport regulation of two-dimensional receptor-ligand association.||Lining Ju||Biophysical journal||4/12/2015||25863068||10.1016/j.bpj.2015.02.023||More Info|
|A Lupus-Associated Mac-1 Variant Has Defects in Integrin Allostery and Interaction with Ligands under Force.||Florencia Rosetti||Cell reports||3/11/2015||25772353||10.1016/j.celrep.2015.02.037||More Info|