Cellular and Molecular Biomechanics Laboratory

The laboratory of Dr. Cheng Zhu at Georgia Tech

Recent News and Publications

Type Date Title Link
4/2/1998 Determining force dependence of two-dimensional receptor-ligand binding affinity by centrifugation. Full
11/30/1998 Measuring two-dimensional receptor-ligand binding kinetics by micropipette. Full
3/9/1999 Probabilistic modeling of shear-induced formation and breakage of doublets cross-linked by receptor-ligand bonds. Full
1/6/2000 Tyrosine replacement in P-selectin glycoprotein ligand-1 affects distinct kinetic and mechanical properties of bonds with P- and L-selectin. Full
2/7/2000 A modified Boyden chamber assay for tumor cell transendothelial migration in vitro. Full


Principal Investigator

Dr. Cheng Zhu



Dr. Hyun-Jung Lee


Graduate Students

Visiting Scholars

Laboratory Alumni

Dr. Jingsung Hong


Laboratory Staff

News From The Zhu Lab


Molecular and Biomechanical Approach in Hematology

Platelet is a key player in both hemostasis and thrombosis. Mechanical stimuli are being identified as important as biochemical cues in the hemodynamic environment of the circulation system. We are solving the puzzles of platelet activation and hyperreactivity by characterizing platelet receptor binding kinetics and signal transduction under forces.

Single Receptor-Ligand Adhesion and Molecular Force Spectroscopy on Cells

Welcome to the Zhu lab. We study a variety of sciences using quantitative, ultra-sensitive tools.

linking cell surface receptor-ligand kinetics with intracellular signaling

T lymphocytes display a remarkable ability to evaluate differences in MHC-peptide complexes. Alteration of a single amino acid in an antigenic peptide has significant and distinct biological effects on the T cell recognition. Current research proposes all these events are governed by the TCR-MHC interactions. Using well developed micropipette and confocal microscope techniques, as well as relative cellular and mouse model, specific experiments are being conducted to find the common mechanism of the TCR-MHC interaction by quantitatively measuring the binding kinetics and comparing with corresponding biological effects, thereby providing a basis for rational design of immunotherapy.


Atomic force microscope


Home-made AFM that measures protein-protein binding kinetics

Biomembrane Force Probe


Measures single ligand-receptor interactions under force

Horizontal atomic force microscope


AFM on which the cantilever works horizontally (parallel to the earth); can measure receptor-ligand binding kinetics under force on live cells

Micropipette adhesion frequency assay

Measures adhesion frequency of single receptor-ligand interactions

Micropipette forge

Home-made micropipette factory: makes pipettes of various sizes


Title First Author Journal Date PMID DOI More Information
Fluorescence Biomembrane Force Probe: Concurrent Quantitation of Receptor-ligand Kinetics and Binding-induced Intracellular Signaling on a Single Cell Yunfeng Chen J Vis Exp. 8/4/2015 26274371 10.3791/52975 More Info
Von Willebrand factor-A1 domain binds platelet glycoprotein Ibα in multiple states with distinctive force-dependent dissociation kinetics. Lining Ju Thrombosis research 6/20/2015 26213126 10.1016/j.thromres.2015.06.019 More Info
Pre-TCR ligand binding impacts thymocyte development before αβTCR expression. Robert J Mallis Proceedings of the National Academy of Sciences of the United States of America 6/8/2015 26056289 10.1073/pnas.1504971112 More Info
The cellular environment regulates in situ kinetics of T-cell receptor interaction with peptide major histocompatibility complex. Baoyu Liu European journal of immunology 5/22/2015 25944482 10.1002/eji.201445358 More Info
Transport regulation of two-dimensional receptor-ligand association. Lining Ju Biophysical journal 4/12/2015 25863068 10.1016/j.bpj.2015.02.023 More Info
A Lupus-Associated Mac-1 Variant Has Defects in Integrin Allostery and Interaction with Ligands under Force. Florencia Rosetti Cell reports 3/11/2015 25772353 10.1016/j.celrep.2015.02.037 More Info
Molecular force spectroscopy on cells. Baoyu Liu Annual review of physical chemistry 1/12/2015 25580628 10.1146/annurev-physchem-040214-121742 More Info
Ligand-engaged TCR is triggered by Lck not associated with CD8 coreceptor. Javier Casas Nature communications 11/27/2014 25427562 10.1038/ncomms6624 More Info
DNA-based digital tension probes reveal integrin forces during early cell adhesion. Yun Zhang Nature communications 10/24/2014 25342432 10.1038/ncomms6167 More Info
A generalizable, tunable microfluidic platform for delivering fast temporally varying chemical signals to probe single-cell response dynamics. Loice Chingozha Analytical chemistry 10/10/2014 25254360 10.1021/ac5019843 More Info

Contact Zhu Lab

If you would like to contact the laboratory of Dr. Cheng Zhu, please get in touch with Larissa (E-mail)

For information about web design or web application design used in this website please contact Aaron (E-mail)

The website is administrated by Jiexi Liao (E-mail)